Results
Peptide Synthesis
To determine the AA sequence of IgE- and IgG-binding sites, 37 overlapping peptides spanning αs1-CN were synthesized (shown in Table 1). By synthesizing peptides that were offset from each other by 5 AA, it was possible to identify individual IgE- and IgG-binding epitopes within the larger regions of the αs1-CN molecule. Each peptide was 15 AA long. All peptides were purified by C18 reverse-phase HPLC.