In rohu Labeo rohita, 1500 mg/kg of ASA supplementation adversely affected the growth performance, immunity and disease resistance (Tewary and Patra, 2008). However, 1042 mg/kg of dietary vitamin C, which was nearly 9 times over the minimal requirement of largemouth bass, did not induce any negative effects in tested parameters or toxicological effects.
The overall results in this study suggested that the optimal dietary vitamin C requirement should be 102.6–147.8 mg/kg based on growth and liver storage in juvenile largemouth bass.