using gallic acid as standard phenolic compound (Slinkard and Singleton 1977). For estimation of anthocyanins content, 2 ml of melted ice cream sample was mixed with 2 ml methanol (containing HCl) and then centrifuged at 3,000 rpm for 10 min. The supernatant (1 ml) was added to 9 ml of 1 N HCl. The absorbance was measured at 520 nm. The total anthocyanins content was calculated as (A)×101×18.89(mg/l), where A is the absorbance (Picinelli et al. 1994). Colour intensity
was obtained by using method of Somers and Evans (1977). For this purpose1 g of ice cream is previously extracted with 10 ml of 80 % methanol overnight on orbital shaker. Absorbance of 1:1 diluted ice cream was recorded at 420 nm, 520 nm and 620 nm on UV spectrophotometer. Colour intensity was calculated by following formula: Colour intensity ¼ ðabs 420 nmÞ þ ðabs 520 nmÞ þ ðabs 620 nmÞ df , where df is dilution factor. Antioxidant activity was measured in form of DPPH assay (Arnous et al. 2001). The method of Lowry (Lowry et al. 1951) was followed for estimation of protein content.