The sticky sorghum syrup was diluted by DDW and quantified by HPLC as described below. Diluted syrup that was autoclaved was then added to different flasks at different volumes to achieve different sugar concentrations. Total sugar concentrations ranging from 12 to 34 g/L were tested. Size of inoculum was always 10% of the final total volume. All cultures were grown in 250-mL Erlen- meyer flasks with a final volume of 100 mL on a rotary shaker set at 150 rpm at room temperature. At different time points, 10 mL samples were withdrawn under sterile conditions and analyzed as illustrated in the following. The same experiment was conducted twice to evaluate the reproducibility of the obtained results.