2.4. Statistical analysis
Fatty acid data (individual, major groups and total muscle fat) were
analysed using analysis of variance (ANOVA) in GenStat (GenStat Release 13.1, Copyright 2010, VSN International Ltd). Analysis was by a
2 × 2 factorial design with two levels of flaxseed flakes (with and without) and two levels of algae (with and without). The treatment structure was specified with fully factorial effects for the factors Flax (Flax
and Nil) by Algae (Algae and Nil). Blocking structure was specified as
Pen (animals were maintained in pens as groups). Graphs of residuals
versus fitted values were used to check distributional normality assumptions for all the variables compared such as muscle vitamin E,
major fatty acid groups, and TBARS. Treatment effects on retail display
colour traits were compared using day of display as repeated measures.
F-tests were used to determine the overall significant difference among
the predicted means, whereas the difference between two predicted
means was judged to be significant if it was at least two times the average standard error of difference (SED).
2.4. Statistical analysisFatty acid data (individual, major groups and total muscle fat) wereanalysed using analysis of variance (ANOVA) in GenStat (GenStat Release 13.1, Copyright 2010, VSN International Ltd). Analysis was by a2 × 2 factorial design with two levels of flaxseed flakes (with and without) and two levels of algae (with and without). The treatment structure was specified with fully factorial effects for the factors Flax (Flaxand Nil) by Algae (Algae and Nil). Blocking structure was specified asPen (animals were maintained in pens as groups). Graphs of residualsversus fitted values were used to check distributional normality assumptions for all the variables compared such as muscle vitamin E,major fatty acid groups, and TBARS. Treatment effects on retail displaycolour traits were compared using day of display as repeated measures.F-tests were used to determine the overall significant difference amongthe predicted means, whereas the difference between two predictedmeans was judged to be significant if it was at least two times the average standard error of difference (SED).
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