Therefore, it is
possible that the Cel3B protein exists in the culture filtrate and
contributes to cellulose saccharification together with Cel3A, the
main b-glucosidase in T. reesei. Because of its identification as the
closest homolog of Aspergillus b-glucosidases with a high cellobiase
activity, we decided to test whether TrCel3B, heterologously
expressed in S. cerevisiae, could compensate for the lack of cellobiase
activity in the T. reesei extract used for hydrolysis of lignocellulosic
biomass in the production of bioethanol from waste
wood.