Algae are one of the major concerns on the bio-fixation of greenhouse gas CO2and high rate pond (HRP) isthe most efficient algal biomass production facility. This study tried to evaluate the potential of CO2miti-gation by algae in the HRP with natural water medium which fits the ecological engineering concept. Theresults showed that the uptake rate could reach 162 mg/L/day and the consumption efficiency was keptup to 123%. Furthermore, through all our study the consumption efficiency remained almost constant. Inaddition, after the comparisons between our study and literature, we demonstrated (1) the mixed cultureperformed better than pure culture, (2) the natural medium absorbed CO2more than artificial mediumand (3) consumption efficiency was more sensitive than uptake rate. Finally, the algal HRP showed thepotential of CO2sequestration and algae might be an important alternative to reduce the greenhouse gasof atmospheric CO2.© 2016 Elsevier B.V. All rights reserved.