Virus
The virus used for the present study was the
CyHV-2 Saitama-1 (SaT-1) isolate which was isolated
in GFF cells from diseased calico goldfish in Saitama
Prefecture, Japan, in 1999. The original virus was
passed once through goldfish at 24.1°C by intraperitoneal
injection into 6 individuals of Wakin goldfish
(average body weight 12.2 g) obtained from a re -
tailer. After the injection, 1 fish died on Day 7 and
another on Day 12. The homogenate of pooled kidney
from both dead fish was inoculated onto monolayers
of GFF cells at 20°C for re-isolation of the
virus. CyHV-2 SaT-1 isolate recovered from these
cultures was used for further studies.