when it demonstrated moderate to high count
values in the body
fluid or tissue of interest with little to no counts
in non-target body
fluids. An example of the expression data
obtained for a single source blood sample is shown in Fig. 1. As can
be seen, high count values are observed for each of the eight
included blood biomarkers with little to no expression of the other
thirty non-blood biomarkers in the multiplex. Therefore, using this
multiplex assay a blood sample could easily be distinguished due
to the high specificity of the included blood-specific biomarkers.
Modifications were made to each subsequent multiplex system in
order to remove biomarkers with poor specificity or sensitivity