Fertile eggs (n=400), forming the broiler breeders flock (Ross 308; broiler breeders age, 40 weeks, first cycle of production, average egg weight, 61.5 g, and production percentage, 79%) maintained on the breeders adequate nutritional plan were collected, weighted and distributed into four groups of 100 eggs. Fertility was verified by candling with a hand ultraviolet lamp at 12th day of incubation. On 14th day of incubation, two groups were injected with 15 or 30 mg/egg VE using 25 mm needle as standardized method[ 14]. Rest two groups were used as sham control (IOI of 0.5 mL sterile physiology serum/egg) and un-injected control. Sterile physiology serum was included as sham control, primarily to rule out a possible negative response caused by the stress of injection and handling. The injections were carried out under laminar flow system, where temperature of the chamber was maintained at 37 °C for avoiding any temperature stress for chicken embryo. Prior to IOI, the site of injection was disinfected with 70% ethanol and the solutions were warmed to 30 °C. The injected eggs were returned to the incubator after injection. Within 20 min, IOI of each treatment was completed of taking out from incubator. Immediately after the injection, the pinhole site was sealed with sterile paraffin wax and eggs were returned to the incubator. On the 19th day of incubation, the eggs were shifted to the hatcher and kept in the respective pedigree hatching boxes. On the day of hatch, chickens were weighted and hatching percentage was recorded.