2.5. Sequencing
All PCR fragments in meat adulteration experiments were purified
using the Gel-M™ Gel Extraction System (Viogene, Taiwan),
and sequencing was conducted at the Genomics BioSci & Tech
Company using a 3730xl DNA Analyzer (Applied Biosystems).
The nucleotide sequences were assessed for sequence similarity
through a BLASTn search using the National Centre for
Biotechnology Information database, followed by alignment and
100% matching with the chicken, pork, goat, beef and ostrich
sequences available in the GenBank database.