In this study, D101 resins were packed into a column and used to
crudely separate GAMG from fermentation broth. The dynamic absorption
capacity of GAMG was investigated at three loading flow rate: 0.5, 1
and 1.5 ml·min−1
. As can be seen from Fig. 7(a), the leaked amount of
GAMG in the outlet increased as the increase of flow rate. This result
was reasonable since the flow rate directly affected the diffusion of
GAMG into the internal surface of the resin. When flow rate was too
high, the contact time between GAMG and D101 was too short so that
GAMG cannot efficiently diffuse into the internal space of the resin. At
flow rate of 0.5 ml·min−1
, the maximum absorption capacity achieved
to be 76.1 mg·g−1 resin, indicating that the loading flow rate should not
be too high in order for preventing the leak of GAMG (Table 2). Therefore,
0.5 ml·min−1 was chosen as the optimal loading flow rate. The absorption
capacity reported here was higher than the references. For
example, Guo et al. used D101 resin to separate puerarin from Puerariae
Lobatae Radix with absorption capacity of 51.4 mg·g−1 [31]. D101 resin
has also been applied in the separation and purification of amygdalin
from bayberry, and the absorption capacity was around 33.0 mg · g−1
[32]. The difference in absorption capacity should be attributed to the
different characteristics of the target compounds, and comparison also
indicated that D101 resin was suitable for the preliminary separation
of GAMG