2.6. Immunological procedures 2.6.1. Blood collection Groups of CD1 female mice with 20–25 g were injected intraperitoneally with 1 mg/animal of Bxb, aspirin or dexamethasone. Control mice received 0.1 mL of saline solution. After 0.5, 1, 2, 4, 6, 12 and 24 h of injection with Bxb, aspirin or dexamethasone animals were bled by the retro orbital plexus. The blood samples were allowed to stand until they formed a clot and the sera were used in cytokines analysis.