Samples were spotted and run with chloroform/methanol/acetic
acid (285:21:9) as solvent system. The Monascus pigments were
detectable under visible light. The spots of Monascus pigments
appeared at different locations, i.e., red Monascus pigments at origin,
yellow ones at Rf = 0.73, and orange ones at Rf = 0.95 [12].