2.3. Measurement of total phenolic content
The total phenolic content (PC) was analysed each time the
chicken samples were processed to determine the volume of grape
residue extract that needed to be added in the meat (the volume was
calculated to obtain a concentration of 60 mg PC/kg of meat). The
analysis was performed in triplicate. Total phenolic content of IGE and
NGE was determined colorimetrically through the method proposed
by Singleton, Orthofer, and Lamuela-Raventos (1999). The extract
solutions (0.1 mL) were mixed with 5 mL of distilled water and 0.5 mL
of Folin-Ciocalteau reagent. After 3 min of reaction, 1.5 mL of Na2CO3
(20%) and 2.9 mL of distilled water were added. The absorbance was
measured in a spectrophotometer (Shimadzu UV-1240, Chiyoda-ku,
Tokyo, Japan) at 765 nm after 2 h of incubation in the dark at room
temperature. PC were expressed in gallic acid equivalents (GAE) per
100 g (dry weight) of grape residue.
2.3. Measurement of total phenolic contentThe total phenolic content (PC) was analysed each time thechicken samples were processed to determine the volume of graperesidue extract that needed to be added in the meat (the volume wascalculated to obtain a concentration of 60 mg PC/kg of meat). Theanalysis was performed in triplicate. Total phenolic content of IGE andNGE was determined colorimetrically through the method proposedby Singleton, Orthofer, and Lamuela-Raventos (1999). The extractsolutions (0.1 mL) were mixed with 5 mL of distilled water and 0.5 mLof Folin-Ciocalteau reagent. After 3 min of reaction, 1.5 mL of Na2CO3(20%) and 2.9 mL of distilled water were added. The absorbance wasmeasured in a spectrophotometer (Shimadzu UV-1240, Chiyoda-ku,Tokyo, Japan) at 765 nm after 2 h of incubation in the dark at roomtemperature. PC were expressed in gallic acid equivalents (GAE) per100 g (dry weight) of grape residue.
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