2.8. Statistical analysis
Statistical analysis of the results was performed using the KolmogoroveSmirnov
test to verify that the data had a normal distribution
and by the Levene test to check homogeneity of variance.
Average values for fungal colonization and fumonisin content
were log-transformed, while percentage values derived from
counting were arcsine-transformed before statistical analysis. Statistical
analysis of the effect of T. harzianum on F. verticillioides
incidence and fumonisin content was performed using ANOVA
followed by Tukey's HSD test, in which treatment (T), year (Y) and
site (S) were independent variables. Pairwise comparisons of
T. harzianum and F. verticillioides colonization (CFU∙g1
) in roots
and silks between treated and untreated plants were conducted
using Student's t-test, analyzing year and site. Comparisons of
T. harzianum root colonization in different years and growth stages
were performed using ANOVA. The analyses were conducted using
the software R version 3.0.2 (http://www.rproject.org).