2.2. Cultivation of Acetobacter xylinus 23769Two types of culture media were used: (1) the standardHestrin–Schramm culture medium (HS) (2 w/v% glucose, 0.5 w/v%bacto peptone, 0.5 w/v% yeast extract, 0.115 w/v% magnesium sul-fate heptahydrate and 0.27 w/v% disodium hydrogen phosphate)and (2) BC-wood hot water extraction medium. For the preculture,the stock culture was inoculated into 50 ml of the HS medium ina 250 ml Erlenmeyer flask and culture was allowed to proceed at