Limit of detection (LOD) or the minimum amount ofanalyte which can be detected reliably is a peak whichits signal-to-noise ratio is at least 3:1. The limit ofquantification (LOQ) or minimum quantifiable amountis the concentration which can be quantified with aspecified level of accuracy and is often calculated with asimilar method for LOD whose signal-to-noise ratio is atleast 10:1. But in this study LOQ was defined with Relativestandard deviation (RSD) less than 3, experimentally, byinjecting different concentrations of standard with signalto-noise ratios of 10, 15, 20, and 25 (13-15).