We have successfully constructed a sensitive and selective electrochemical sensor for the determination of neomycin. The sensor was fabricated via stepwise modification of CS-SNP and GR-MWCNTs composites and a MIPs film on gold electrode. The excellent performance of the MIPs/GR-MWCNTs/CS-SNP/gold electrode towards neomycin was ascribed to the synergistic effects of the good electrical conductivity of CS-SNP and GR-MWCNTs composites. The electrochemical sensor exhibited