Sake (Japanese rice wine) is made from only a few ingredients, i.e., rice, water, yeast, and a mold, Aspergillus oryzae, known as koji-mold. The starch in the steamed rice is hydrolyzed into glucose by the cooperative action of saccharification enzymes such as α-amylase and glucoamylase that are produced by A. oryzae. The resulting glucose is converted to alcohol by the yeast Saccharomyces cerevisiae during the sake-mash fermentation. The processes of saccharification and fermentation proceed simultaneously through the period of sake manufacturing. Thus, the balance between both processes significantly affects the quality of the sake.