Verification of ESBL producing E. coli
After selection of unsusceptible E. coli via agar dilution
with cefotaxime containing MacConkey-agar (1 μg/mL),
the species was confirmed using MALDI-TOF identification
(MALDI Microflex ® LT and Biotyper ® database,
Bruker Daltonics, Bremen, Germany). Identification of
ESBL encoding genes of the CTX-M, TEM, SHV and
CYM families was carried out by PCR according to the
method published by Roschanski et al. [34].