Raman measurements were made by using a 600-groovesmm1 grating and a confocal microscope with a 100-m aperture, giving a resolution of 4–8 cm1. Spectra were scanned for 60 sec in the 200- to 1,400-cm1 range (Jobin–Yvon LabRam HR 800 microRaman). The ACC precipitate was dissolved with 10 ml of 0.5 M EGTA, 0.02 M ammonium acetate (pH 7.0) and dialyzed. Extracted protein and GAP 65-
enriched fractions were separated by SDS/PAGE