Prior to inoculation, explants of both leaves and rhizomes werewashed with sterile seawater and inoculated in test tubes contain-ing 20 ml of full strength MS (Murashige and Skoog, 1962) gelled(0.8% agar, w/v) medium supplemented with 3% sucrose. Differ-ent combinations of the auxin 2,4-D with the cytokinins BAP andKinetin at different concentrations (0.5, 1 and 2 mg l−1) were tested