The same wet gluten as in Example 1 was freeze-dried,
subsequently ground, and sieved, thereby obtaining a vital gluten.
The vital glutens obtained in Example 1, Comparative Examples 1 to 3, and Control Group 1 were each added to distilled ,water with stirring to give a concentration of 10%, thereby evaluating the water การกระจายตัวได้.
15 Next, the vital gluten was added to distilled water to give a
concentration of 35% to be made to become like a wet gluten, and 7 panelists conducted a sensory evaluation on flavor, color and gel physical properties. The results are shown in Table 2.
The criteria for water การกระจายตัวได้ evaluation of the vital
20 glutens are also shown in Table 3. The gel physical property having
สมบัติหยุ่นหนืด was considered good, and evaluated based on Control Group 1.
[0048] (Table 2)
In-สารละลายการกระจายตัว Water-soluble pea
preparation method โพลีแซคคาไรด์
(สารละลาย added Water-soluble soybean preparation method) โพลีแซคคาไรด์
Acetic acid
Ammonia
Properties of การกระจายตัวได้
Wet 10% dispersion pH
gluten สารละลาย
Viscosity (mPa.sec) Properties of
10% dispersion การกระจายตัวได้
สารละลาย
35% Gluten การกระจายตัวได้
Flavor
Example 1
0,05%
สารละลาย
_
-
-
4
6.5
18
4
1
A
Comparative
Example 1
-
สารละลาย
-
-
4
6.5
24
4
1
B
Comparative
Example 2
-
Adjusted to
pH 4,6
-
4
4.6
530
2
'1
C: foul taste,
Example '3e
-
Adjusted to
pH 9,3
4
9,3
80
2
'1
C: Foul smelt
Control
Group 1
-
-
1
-
-
1.5
1
A
Vital
gluten
Color
- foul smell
A A 13: Slightly D: Yellow A
yellow
B
Gel physical properties A Slightly low
elasticity
D
Low
stickiness,
low elasticity
D
Low A
stickiness
Flavor evaluation/Color evaluation/Gel physical properties: A Very good, B Good, C Rather poor, D Very poor
[0049] (Table 3)
Water การกระจายตัวได้ Evaluation Criteria
4: Free of agglomerates, readily dispersed
3: Dispersed but gluten agglomerates are partially noted 2: Many gluten agglomerates are noted, hardly dispersed 1: A gummy lump is formed, not dispersed at all
[0050] When the เวเว of Example 1 were added, the wet gluten was dispersed satisfactorily at a neutral pH,
5 providing a low viscosity สารละลายการกระจายตัว, and the vital gluten was
obtained more efficiently by spray drying. Further, the obtained vital gluten was free of a foul smell and had a good color. Furthermore, the
24
FP15-0572-00
vital gluten, when added to and mixed with water to give a concentration of 10%, was dispersed satisfactorily. The vital gluten, when added to water to give a concentration of 35%, became like an
elastic wet gluten. The obtained gluten had a good flavor and color
5 with the texture distinctive to gluten despite a lightly lower
สมบัติหยุ่นหนืด compared with Control Group.
[0051] When the water-soluble soybean โพลีแซคคาไรด์ of Comparative Example 1 were added, the wet gluten was dispersed satisfactorily at a neutral pH but the สารละลายการกระจายตัว had a higher
10 viscosity than Example 1. A vital gluten was also obtained despite
poorer spray properties at the time of spray drying than Example 1. The obtained vital gluten was free of a foul smell and had a good color despite a slight soybean flavor noted. Further, the vital gluten, when added to and mixed with water to give a concentration of 10%, was
15 dispersed but poorer than Example 1. The vital gluten, when added to
water to give a concentration of 35%, became like an elastic wet gluten. The obtained gluten had a poorer flavor than Example 1 but had a good color with the texture distinctive to the gluten, despite the lower สมบัติหยุ่นหนืด compared with Control Group.
20 Thus, when the เวเว were used in
the system where a gluten concentration is 10% in the สารละลายการกระจายตัว, the viscosity was reduced by 25% compared with the case where the water-soluble soybean โพลีแซคคาไรด์ were used, consequently achieving a significant improvement in workability of the
25 สารละลายการกระจายตัว.
[0052] The gel containing 35 wt% of the vital gluten of Comparative
25
FP15-0572-00
Example 2 had a foul smell, developed a slightly acidic taste, and showed discoloration of a reddish color. The gel was low in both stickiness and elasticity, and soft.
Likely, the gel containing 35 wt% of the vital gluten obtained in
5 Comparative Example 3 had an intense foul smell, and developed a
color with yellow to reddish discoloration. The gel also had a low stickiness
[0053] The wet gluten of Control Group 1 was added to water to give a concentration of 10% but was not dispersed at all and formed an
10 agglomerate. The vital gluten also had strong aggregability and was
hardly dispersed in water, hence failing to prepare a 10% สารละลายการกระจายตัว.
[0054] (Example 2: Comparison of vital gluten water การกระจายตัวได้ by the amount of เวเว added)
15 The effective concentration. of water-soluble pea
โพลีแซคคาไรด์ for gluten dispersion was checked. เวเว were added to distilled water at 20°C and stirred, thereby obtaining a สารละลาย. Note that the concentration of
สารปรับปรุงการกระจายตัวได้ shown in Table 4 was expressed by wt% when
20 the vital gluten was 100 wt%. The vital gluten (a solid content 95.5%)
obtained in Control Group 1 was added thereto to give a concentration of 10 wt% while stirring (500 rpm) using a three one motor (SHINTO Scientific Co., Ltd.), thereby evaluating the การกระจายตัวได้ of the gluten.
The การกระจายตัวได้ was evaluated based on the criteria shown in Table 3.
25 Further, of the 10% vital สารละลายการกระจายตัว ของกลูเตนs, those recognized to
have the การกระจายตัวได้ were measured for the median diameter of gluten
26
FP15-0572-00
particles using SALD-2000A (Shimadzu Corporation). The result of the comprehensive evaluation based on the 3 items of การกระจายตัวได้, viscosity and median diameter was defined as overall evaluation. The results are shown in Table 4.
5 [0055] (Comparative Example 4: Comparison of vital gluten water
การกระจายตัวได้ by the amount of water-soluble soybean โพลีแซคคาไรด์ added)
The water การกระจายตัวได้ of the vital gluten was evaluated exactly in the same manner as in Example 2, with the exception that
10 water-soluble soybean โพลีแซคคาไรด์ were used in lieu of the
เวเว. The results are shown in Table 4. [0056] (Control Group 2: Water การกระจายตัวได้ of vital gluten)
The water การกระจายตัวได้ of the vital gluten was evaluated without adding the สารปรับปรุงการกระจายตัวได้ unlike in Example 2. The
15 results are shown in Table 4.
[0057] (Table 4)
Control
Example 2 Comparative Example 4 Group
2
Kind of Water-soluble
additive pea
โพลีแซคคาไรด์ A
(%)
Water-soluble soybean
โพลีแซคคาไรด์
(%)
Evaluation Dispersibiltty
result Viscosity
(mPa.sec)
Median diameter
(µm)
Overall evaluation
0.005 0.01 0.05 0.1 1 5 10 -
- - - - - - - 0.005
3 4 4 4 4 4 4 1.5
38.5 10.5 9.0 9.0 8,5 8.5 13.5 38,5
184 85 40 25 34 41 45 295
D B A A A A A D
- - - - - - -
0,01 0.05 0.1 1 5 10 -
2 3 4 4 4 4 1.5
30.5 27.5 18,5 9.5 10.5 26.5 -
2B4 117 74 65 28 42 -
D C A .A A A
Overall evaluation: A Very good, B Good, C Rather poor, D Very poor
[0058] The results shown in Table 4 revealed that the water-soluble pea
27
FP15-0572-00
โพลีแซคคาไรด์ dispersed the vital gluten satisfactorily in an amount added of 0.01% or more. On the other hand, the water-soluble soybean โพลีแซคคาไรด์ caused slightly insufficient dispersion and the formation of a gummy lump in an amount added of 0.05% or less, but
5 dispersed the vital gluten satisfactorily in an amount added of 0.1% or
more. Satisfactory การกระจายตัวได้ was achieved by increasing an
amount of the การกระจายตัวได้ สารปรับปรุง depending on the gluten concentration. When an amount added was too large, a viscosity of สารละลายการกระจายตัว tends to increase but, when an. amount added was
10 adjusted, the สารละลายการกระจายตัว with good การกระจายตัวได้ and a low
viscosity was obtained.
These results verified that the เวเว can satisfactorily disperse the vital gluten in a lower viscosity in a smaller amount added compared to the water-soluble soybean
15 โพลีแซคคาไรด์.
[0059] (Example 3: Fish meat sausage)
44 g of salt, 220 g of lard, 60 g of sugar, 36 g of seasoning, 140 g of cornstarch, and 40 g of the vital gluten obtained in Example 1 were dispersed in 880 g of chilled water and added to 2000 g of minced
20 Alaska pollock, and mixed using a silent cutter, thereby obtaining a fish
meat paste. Subsequently, the obtained fish meat paste was packed in a casing, sealed, and braised at 95°C for 20 minutes. Then, cooling was carried out in chilled water and the paste was stored in a refrigerator overnight to carry out the sensory evaluation.
25 [0060] (Comparative Example 5)
A fish meat sausage was obtained exactly in the same manner as
28
FP15-0572-00
in Example 3, with the exception that the vital gluten obtained in Comparative Example 1 was used.
[0061] (Control Group 3)