Rapid propagation by
65 the shoot generation calli was achieved in polyvinylpyrrolidone
66 (PVP) and growth regulator supplemented MS medium (Roy
67 and Sarkar, 1991). However, no attempt has yet been made
68 to quantify barbaloin (1) comparatively in natural or in vitro
69 populations of A. vera. Since barbaloin is considered as an
70 important biologically active compound in A. vera, the present
71 investigation depicts a validated HPTLC protocol for determina-
72 tion of barbaloin from in situ and in vitro grown populations of A.