During exponential growth, MurG has been shown to localize in a FtsZ-dependent manner to septa of C. crescentus, a zone of active peptidoglycan synthesis (Aaron et al., 2007). In B. subtilis MurG-GFP expressed during exponential growth also localized to septa (JDB2501) (Fig. 3A). MurG-GFP signal from the V74E point mutant also localized tothe septum duringexponential growth (Fig. 3A) and protein levels were as wild type (Fig. S2). None of the point mutations showed lethality and cells expressing MurG-GFP point mutants grew normally in absence of the wild type murG allele (Fig. 3B). Thus, MurG localizes to zones of peptidoglycan synthesis during exponential growth and its N-terminal amphipathic helix is dispensable.