Mycotoxin contamination is a common problem on feedstuffs, that can be formed on crops in the field,
during harvest, storage, processing or feeding. The scope of the current study was to investigate the
levels of Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Diacetoxyscirpenol, Ochratoxin A, Toxin
HT-2, Toxin T-2 and zearalenone in a variety of feedstuffs (maize silage, alfalfa hay, cottonseed cake, corn
grain and concentrates) fed to ruminants and the possible contamination of milk though consumption.
For this purpose an easy and simple multiresidue LC–MS/MS method without any clean-up step was
developed and successfully validated in feed and milk matrices. The LOQ of the method was set at 10 μg/
kg for all analytes and 0.05 μg/kg for Aflatoxin M1 and Ochratoxin A in milk. The results showed that
7 cottonseed cake samples, out of 13 were contaminated with Aflatoxin B1 at a level higher than the
maximum levels as set by EU Regulations and with Toxin T-2 with values ranging from 8 to 562 μg/kg.
Nine maize silages and 6 alfalfa hay samples were contaminated with Aflatoxin G2 at levels higher than
the maximum tolerance limit. No mycotoxins or their metabolites were found above the LOQ in any of
the analyzed milk samples.
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Mycotoxin contamination is a common problem on feedstuffs, that can be formed on crops in the field,
during harvest, storage, processing or feeding. The scope of the current study was to investigate the
levels of Aflatoxin B1, Aflatoxin B2, Aflatoxin G1, Aflatoxin G2, Diacetoxyscirpenol, Ochratoxin A, Toxin
HT-2, Toxin T-2 and zearalenone in a variety of feedstuffs (maize silage, alfalfa hay,เค้กเมล็ดข้าวโพด
เมล็ดพืชและเข้มข้น ) เลี้ยงปศุสัตว์และการปนเปื้อนที่เป็นไปได้แม้ว่าการบริโภคนม .
เพื่อง่ายและ LC MS / MS multiresidue ง่าย–วิธีโดยไม่ต้องทำความสะอาด ขั้นตอนนี้ถูกพัฒนาและตรวจสอบเรียบร้อยแล้ว
ในอาหารและนม เมทริกซ์ โดย loq ของวิธีการที่ถูกกำหนดไว้ที่ 10 μ g /
กก. สารทั้งหมด 005 μg/kg for Aflatoxin M1 and Ochratoxin A in milk. The results showed that
7 cottonseed cake samples, out of 13 were contaminated with Aflatoxin B1 at a level higher than the
maximum levels as set by EU Regulations and with Toxin T-2 with values ranging from 8 to 562 μg/kg.
Nine maize silages and 6 alfalfa hay samples were contaminated with Aflatoxin G2 at levels higher than
ขีดจำกัดความอดทนสูงสุด ไม่มีไมโคท็อกซินหรือสารของพวกเขาถูกพบในใด ๆข้างต้น loq
&วิเคราะห์ตัวอย่างนม
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