The activity of LDH was determined using commercially available
kit (BioVision, USA).19 The standards and samples were
prepared following manufacturer’s instruction. The intensity
of coloured product (NADH) was measured using VERSA
micro plate reader (USA) at 450 nm. OD 450 nm was measured
to read A1 and incubated at 37 C for 30 min. Following the
incubation OD 450 nm was measured again to read A2. The
standard curve was generated using the A2 reading after subtracting
the blank reading. Values of NADH at A1 and A2
were calculated from the standard curve, and the LDH activity
calculation was based on the manufacturer’s instruction: