2.4. Immune parameters
At the end of the feeding trial, three fish from each tank (i.e.
3 3 ¼ 9 fish per group) were collected to study the immune
parameters. Blood samples were collected by caudal venipuncture
using a 1-mL syringe after anesthetizing the fish with diluted
MS222 (SigmaeAldrich, USA). The blood samples were transferred
into eppendorf tubes. After collection, blood was centrifuged at
2000 g at 4 C for 10 min. Obtained blood leucocytes and plasma
was stored at 20 C for further analysis.