Carbohydrates were extracted from the powdered frozen tis-
sue in successive steps with different ethanol/water solutions
according to Agüera et al. (2006). The supernatants from the cen-
trifugations were collected and combined for the analysis of soluble
sugars, saving the pellets for starch determination. Sucrose, glucose
and fructose were determined according to Outlaw and Tarczynski
(1984), Kunst et al. (1984) and Beutler (1984), respectively. The
pellets were resuspended in water and incubated at 100 ◦C for 5 h.
Glucose was then released by incubation with -amylase and amy-
loglucosidase, and assayed enzymatically as described above.