2.3.4. CrystallinityThe crystallinity of acetylated and non-acetylated nanofiberswas recorded on an X-ray diffractometer, Hitachi S-4160 (Japan),with CuK radiation ( = 1.54˚A). The measurements were carriedout in 2 ranges between 5◦and 50◦with step size of 0.026◦andtime per step of 99.45 s. The crystalline index of cellulose, CrI,was calculated based on the empirical method proposed by Segal,Creely, Martin, and Conrad (1959) as follows:
CrI (%)
=
(I002 −
Iam)
I002
×
100 (1)
where I002is the peak intensity corresponding to cellulose I, andIamis the peak intensity of the amorphous fraction.