high performance liquid chromatography,
using an Aminex HPX-87H column (Bio-Rad, Hercules,
CA) and 0.005 M H2SO4 as eluent with a flow rate of
0.4 mL min-1 keeping oven temperature at 65 C, with RI
detector and UV/VIS-detector at 280 nm. Calibration was made with glucose, xylose, arabinose, and acetic acid as
standards. As the chromatograph column does not separate
xylose from mannose and galactose, these three sugars
were accounted as xylose. The global recovery yield of
neutral sugars and acetic acid was close to 90 %. The data
were normalized to 100 % yield, assuming equivalent
losses for all sugars.