-We have developed an efficient and costeffective method for commercial micropropagation of
Smooth Cayenne pineapple.
-In vitro shoots were used as starting materials, and either longitudinal sections of the
shoots or leaf bases were used as the explants to regenerate shoots.
-When these explants were used, the axillary meristems, which usually remain quiescent
during shoot multiplication, were able to form new shoots.
-Subsequent to the regeneration step, additional multiplication was achieved inside a 10-l Nalgene vessel with shoots immersed in liquid medium for 5–10 min/h (periodic immersion bioreactor, PIB).
-The shoots were then induced to form roots and transferred to soil.
-Using the above micropropagation method and the PIB, we produced 6,000–8,000 shoots from two initial shoots in less than 6 months.
-The clonal fidelity of propagated plants was tested in Costa Rican and Indonesian pineapple
farms.