Abstract--Two isolectins (ALA-I and ALA-II), were isolated from seed extracts of Artocarpus lakoocha by anion
exchange chromatography on Q-Sepharose fast flow columns at pH 8.5 and 8.0. ALA-I was unbound to the column at
pH 8.5 and moved towards the cathode in non-denaturing polyacrylamide gel electrophoresis, whereas ALA-II
possessed opposite properties. The two A. lakoocha agglutinins appeared to be composed of two dissimilar subunits (e
and fl of M, 14 000 and 17 200) bound non-covalently. The isolectins possessed several similar properties including:
blood type agglutination; pH optimum; pH and temp stability; as well as binding specificity towards asialomucins.