20 μl, were mixed with 1 ml of diluted ABTS radical solution and incubated at 30 °C.
The absorbance at 734 nm was read every minute for 30 min using the Safire microplate reader.
Ethanol (95%) was used as a blank.
Trolox with concentrations from 0 to 500 μM was used as a standard.
The free-radical-scavenging activity was expressed as μmoles of Trolox per gram of sample (μmol TE/g fw or dw).