(vii) To investigate the reversibility of NB-B-induced inhibition of
flowering by subsequent exposure to FR, plants were subjected
to B-SD (90 mol m−2 s−1), B-SD plus a SNB-B (90 mol m−2 s−1;
B-SD/SNB-B), or B-SD/SNB-B followed by 15 min of FR light
(10 W m−2, 62.5 mol m−2 s−1; B-SD/SNB-B/FR) (Table 5). The light
conditions used are summarized in Supplementary Fig. S1.
Data collection and analysis
The following measurements were recorded: length of stem and
leaf number at the start and end of experiments; days until flower
buds became visible; and number of nodes per shoot at flowering.
If no flower buds were visible by the end of the experiment,
shoot tips were dissected under a stereoscopic microscope, and
the number of leaf primordia and floral stage were recorded. The
stage of floral development of each plant was indicated as follows:
stage 0, vegetative shoot apex; stage 1, dome-shaped stage; stage
2, first stage of involucre formation; stage 3, final stage of involucre