2.5. Supercritical carbon dioxide (SC-CO2) extraction using various techniques
The SFE apparatus consisted of two intelligent HPLC pumps (model PU-1580, Jasco Corporation, Tokyo): a CO2 pump and a cosolvent pump. The CO2 pump was fitted with a cooling jacket to deliver CO2, and the cosolvent pump was fitted to deliver cosol- vents as modifiers/entrainers. CO2 from the supply tank was cooled using a cooling jacket attached to the Intelligent HPLC pump. To cool the pump head, an ethylene glycol–deionised water mixture (50:50, v/v) was circulated through the cooling jacket using a
low-temperature bath circulator (model 631D, Tech-Lab Manufac- turing Sdn. Bhd., Selangor, Malaysia), which can chill coolant down to