The results obtained from the disc diffusion method showed the effect of CPL-AgNPs, CPL extract and ampicillin on both Gram positive and Gram negative bacterial strains. The zone of inhibitions measured for different bacterial strains were 7 mm and 9 mm for M. luteus at 5 μg/mL and 10 μg/mL of CPL-AgNPs, and in case with CPL extract and ampicillin alone it was 5 mm and 16 mm, respectively. S. aureus showed 8 mm and 11 mm upon treatment with 5 μg/mL and 10 μg/mL of CPL-AgNPs, and in case with CPL extract and ampicillin alone it was 6 mm and 14 mm, respectively. For B. subtilis it was observed to be 7 mm and 11 mm for CPL-AgNPs, and for CPL extract and ampicillin alone it was 5 mm and 14 mm. E. coli showed zone of inhibition of 7 mm and 13 mm when treated with 5 μg/mL and 10 μg/mL of CPL-AgNPs and with CPL extract and ampicillin it gave 5 mm and 13 mm. P. putida showed 6 mm and 10 mm with 5 μg/mL and 10 μg/mL of CPL-AgNPs and with CPL extract and ampicillin it was observed to be 4 mm and 15 mm. K. pneumoniae showed zone of inhibition of 7 mm and 9 mm when treated with 5 μg/mL and 10 μg/mL of CPL-AgNPs and with CPL extract and ampicillin it was 4 mm and 14 mm. The concentration of CPL extract (5 μg/mL) and ampicillin (10 μg/mL) was kept constant throughout the experiment (Fig. 8); Table 1. The turbidimetry analysis of CPL-AgNPs showed good antibacterial activity with MIC and MBC of 100 μg/mL for S. aureus and E. coli; B. subtilis and K. pneunominae showed MIC and MBC of 50 μg/mL; P. putida and M. luteus were more sensitive to the extract with MIC and MBC of 25 μg/mL.