Many types of commercial vegetable oils were initially used to find an appropriate substrate for Bacillus sp.
GY19. The most suitable substrate found to be corn oil, olive oil, palm oil and soybean oil. In order to reduce
the cost of biosurfactant production, wastes from these vegetable oil production processes were used as
substrate. However, corn oil and olive oil are not the main agricultural product in Thailand, thus only palm
oil and soybean oil were considered. Palm oil mill effluent gave lower biosurfactant production than soy
molasses. It was probably because palm oil mill effluent contained toxic phenolic compounds, while soy
molasses contained mainly saccharides and some residual fatty acids. The determination of optimal condition
and concentration of oil mill effluents revealed that 20% (w/v) of soy molasses gave the highest crude
biosurfactant produced within 120 hr. Moreover, the produced biosurfactant could reduce the surface tension
of medium from 64 to less than 40 mN/m and caused emulsification against diesel oil over 65%. Thus, this
surface active substance could be useful for remediation of insoluble contaminants in the environment.
Many types of commercial vegetable oils were initially used to find an appropriate substrate for Bacillus sp.GY19. The most suitable substrate found to be corn oil, olive oil, palm oil and soybean oil. In order to reducethe cost of biosurfactant production, wastes from these vegetable oil production processes were used assubstrate. However, corn oil and olive oil are not the main agricultural product in Thailand, thus only palmoil and soybean oil were considered. Palm oil mill effluent gave lower biosurfactant production than soymolasses. It was probably because palm oil mill effluent contained toxic phenolic compounds, while soymolasses contained mainly saccharides and some residual fatty acids. The determination of optimal conditionand concentration of oil mill effluents revealed that 20% (w/v) of soy molasses gave the highest crudebiosurfactant produced within 120 hr. Moreover, the produced biosurfactant could reduce the surface tensionof medium from 64 to less than 40 mN/m and caused emulsification against diesel oil over 65%. Thus, thissurface active substance could be useful for remediation of insoluble contaminants in the environment.
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