The optimum conditions, which yielded a conversion of palm oil of nearly 92 and 94% for duck and chicken eggshell waste-derived catalyst, were reaction time 4h, reaction temperature 65°C, methanol/oil molar ratio 9, and catalyst loading 20 wt.% with pressure 1 atm in glass reactor. The experimental results show that CaO catalyst had excellent activity and stability during transesterification. The catalyst was used for 4 times and apparent low activity loss was observed. The fuel properties of the biodiesel so obtained meet all biodiesel standards. As a solid catalyst, CaO can decrease the cost of biodiesel and the steps of purification. It has potential for industrial application in the transesterification of palm oil to FAME.