Erlenmeyers flasks containing 100 mL of clarified cashew apple
juice were then inoculated with 7.00 log CFU/mL (1 mL of MRS broth
containing 9.00 CFU/mL of L. casei). This concentration was chosen
based on the recommendation for probiotic foods: minimal counts of
7.00 Log CFU/mL for better efficacy in regulating beneficial effects
(Vinderola & Reinheimer, 2000).