B. bassiana were grown in media containing 40 or 200 g
glucose L−1 and incubated at 350 or 175 rpm agitation speeds.
These glucose concentrations were known to significantly affect
blastospore yield as well as the osmotic pressure of the
culture medium. Using this full factorial experiment, DO
levels were continuously monitored with blastospore yield
and biomass accumulation measured after 2 and 3 days
growth. The time of fermentation required to reach 50 %
DO (DO50) was also recorded.