Artificial intestinal juices
Artificial intestinal juices used in the model for in vitro study were prepared by dissolution 5 mL of pancreatic extract (4 g/L) and bile salts (25 g/L) in 0.1 M NaHCO3 solution followed by supplementation with quadruple-distilled water to 1000 mL ( Neumann, Goderska, Grajek, & Grajek, 2006).
All artificial digestive juices were checked for Zn content. The determined concentration of this compound in artificial saliva, and gastric and intestinal juices was below 10 μg/L.