BioSafety Journal
BioSafety
Volume 1, Paper 8 (BY95008), June 27th 1995
Online Journal, URL - http://bioline.bdt.org.br/by
Risk assessment of animal cell culture
procedures
G. Stacey
Centre for Applied Microbiology and Research, Salisbury, Wiltshire, SP4 OJG, UK.
E-mail: ecacc@ecacc.demon.co.uk
Received June 1st 1995
Code Number: BY95008
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INTRODUCTION
The generation of continuous cell lines from animal and human tissues has yielded
valuable tools for biological studies. Some of these cell lines have also proved important
hosts for expression of recombinant DNA. The fact that cell culture is used in diverse
disciplines has meant that to a large extent it has evaded specific attention regarding risk
assessment. The primary concern relating to safety in cell culture is that animal cells can
provide a suitable medium in which microorganisms, notably viruses, can multiply. There
are a number of levels at which the culture and manipulation of animal cells should be
considered from the point of view of safe handling. This overview attempts to outline the
type of approach which should be adopted in risk assessment along with some practical
suggestions to promote safe handling of cell cultures.