2.4. Antagonistic inhibition on conidial germination of Colletotrichum
sp.
A mixture of 20 μl of Colletotrichum sp. conidial
suspension (1×106 cells/ml) and 20 μl of bacterial cell
cultures was formed at increasing concentrations (1×108,
2×108, 3×108 and 4×108 CFU/ml). The mixture was then
pipetted onto clean glass slides forming a 0.5-cm-diameter
membrane, and slides were then incubated in moist chambers
for six and a half hours using a minimum of three replicate
slides for each fungal isolate. At the end of the incubation
period, a drop of lactophenol was added to each slide to arrestgermination within the drop. The percent germination in each
fungal isolate was determined by counting a minimum of 100
conidia from each isolate under the microscope and determining
the proportion that had germinated.