Grains were soaked in distilled water (1:5) ratio, till their saturation point was reached, excess water was drained and grains were germinated in a Seed Germinator (Macro Scientific Works Pvt. Ltd, India. Relative humidity 1⁄4 85%) with pre-standardized conditions i.e. 25 C for 48 h.
After germination the seeds were dried in hot air oven at 45 C for 18 h till final moisture content of 9.75 g/ 100 g.
The dried grains both raw and germinated were milled in stone mill and passed through 44 mesh sieve (355 mm) to obtain a uniform size of flour.
The flour was stored in refrigeration (4 C) till further analysis.