Fluorescent dyes have also been employed to directly assess yeast viability in
wine. Use of flow cytometry enables information on cell size and shape to be
obtained by light scattering. In addition cell viability can be assessed directly
using fluorescent dyes to view the metabolic state of yeast and bacteria in wine
(Malacrino, et al. 2001; Boyd, et al. 2003; Chaney, et al. 2006; Herrero, et al. 2006).
Flow cytometry can also be useful if, instead of stains, DNA probes or antibodies
specific for a particular microbial species are used (Graca da Silveira, et al. 2002).
This would allow not only the enumeration of targeted microbes but, coupled with
a live/dead marker, also the percentage of living cells versus dead.
Fluorescent dyes have also been employed to directly assess yeast viability inwine. Use of flow cytometry enables information on cell size and shape to beobtained by light scattering. In addition cell viability can be assessed directlyusing fluorescent dyes to view the metabolic state of yeast and bacteria in wine(Malacrino, et al. 2001; Boyd, et al. 2003; Chaney, et al. 2006; Herrero, et al. 2006).Flow cytometry can also be useful if, instead of stains, DNA probes or antibodiesspecific for a particular microbial species are used (Graca da Silveira, et al. 2002).This would allow not only the enumeration of targeted microbes but, coupled witha live/dead marker, also the percentage of living cells versus dead.
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