the dried powder was extracted with methanol/acetic acid (100/1) at ratio 1:10 (w/v) overnight under agitation the extraction was repeated twice for 1 h. Extracts were combined and evaporated under reduced pressure (40 ํc). The residue was solubilized in water/acetic acid (100:1), and washed thrice with diethyl ether (1:1; v/v). The washing phase was evaporated (W1;2.7% w/w). The aqueous phase was brought to pH 10 with ammonia and extracted thrice with dichloromethane (1:1; v/v). The oganic phases were combined and evaporated under reduced pressuer to yield the alkaloid extract (Alk1;1.9% w/w)