The media tried for earliest culture initiation and shoot regeneration included PGR-free MS basal medium and MS media supplemented with 0.1 to 0.3 mg/l (at an interval of 0.05) 6- Benzyl aminopurine (BAP), 0.1 to 0.3 mg/l (at an interval of 0.05) kinetin and 0.1 to 0.3 mg/l BAP in combination with 0.1mg/l α-naphthalene acetic acid (NAA).