However, Nge et al. (2006) showed that 1 kDa shrimp chitosan was more effective than 10 and 100 kDa chitosans in improving D. phalaenopsis protocorm-liked body culture in vitro, whilst O80 was shown to be the best of the tested chitosan molecules in improving floral production in Dendrobium ‘Eiskul’ (Limpanavech et al., 2008).